WormBase Tree Display for Construct: WBCnstr00014210
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| WBCnstr00014210 | Other_name | Expr9663_Ex | |
|---|---|---|---|
| Summary | [ccb-1::GFP] | ||
| Driven_by_gene | WBGene00000368 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Transcriptional_fusion | ||
| Construction_summary | [ccb-1::GFP] transcriptional fusion. For ccb-1 promoter-driven GFP construct, a 4.2 kb region upstream of ccb-1 ATG was amplified from genomic DNA using the following oligos: 5'- GCGCTCTAGACGTTTCCTGGAAGGTTTCTG-3' and 5' GCGCCCCGGGTTCTGCGGAAAGCCATCTAGC-3'. These primers append XbaI and XmaI restriction sites, respectively. The promoter fragment was inserted into a XbaI and XmaI- digested Fire lab vector pPD95.75. --precise ends. | ||
| Used_for | Transgene_construct | WBTransgene00031146 | |
| Reference | WBPaper00040141 |
