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WormBase Tree Display for Construct: WBCnstr00013845

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Name Class

WBCnstr00013845Other_nameExpr9239_Ex
Summary[enu-3p::enu-3(+)::gfp]
Driven_by_geneWBGene00010362
GeneWBGene00010362
Fusion_reporterGFP
Type_of_constructTranslational_fusion
Construction_summary[enu-3p::enu-3(+)::gfp] translational fusion. The enu-3 translational fusion was made using the outside H04D03.1 primer corresponding to 3 kb upstream of the initiator ATG; 5'-CGTGTAACTCCTTTCATCTTGGCAACCATAGCTCCG-3' and the fusion primer located right before the stop codon of H04D03.1; 5'-AGTCGACCTGCAGGCATGCAAGCTATCGCGTGGCTTGTCCACAGT-3' using LongAmp Taq Polymerase (New England Biolabs). A GFP gene with an unc-54 3' UTR PCR product was made from pPD95.75 using primers; 5'-AGCTTGCATGCCTGCAGGTCG-3' and 5'-AAGGGCCCGTACGGCCGACTA-3'. The two PCR products were then fused using nested primers: for H04D03.1; 5'-CTGTTGAAGGAGATCCTCTTT-3' and for GFP; 5'-GGAAACAGTTATGTTTGGTATA-3' using TaKaRa Ex Taq polymerase (Takara Biotech Inc.). These reactions generated a fusion product of approximately 6 kb called enu-3p::enu-3(+)::gfp.
Used_forTransgene_constructWBTransgene00031024
ReferenceWBPaper00038105