WormBase Tree Display for Construct: WBCnstr00013845
expand all nodes | collapse all nodes | view schema
WBCnstr00013845 | Other_name | Expr9239_Ex | |
---|---|---|---|
Summary | [enu-3p::enu-3(+)::gfp] | ||
Driven_by_gene | WBGene00010362 | ||
Gene | WBGene00010362 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Translational_fusion | ||
Construction_summary | [enu-3p::enu-3(+)::gfp] translational fusion. The enu-3 translational fusion was made using the outside H04D03.1 primer corresponding to 3 kb upstream of the initiator ATG; 5'-CGTGTAACTCCTTTCATCTTGGCAACCATAGCTCCG-3' and the fusion primer located right before the stop codon of H04D03.1; 5'-AGTCGACCTGCAGGCATGCAAGCTATCGCGTGGCTTGTCCACAGT-3' using LongAmp Taq Polymerase (New England Biolabs). A GFP gene with an unc-54 3' UTR PCR product was made from pPD95.75 using primers; 5'-AGCTTGCATGCCTGCAGGTCG-3' and 5'-AAGGGCCCGTACGGCCGACTA-3'. The two PCR products were then fused using nested primers: for H04D03.1; 5'-CTGTTGAAGGAGATCCTCTTT-3' and for GFP; 5'-GGAAACAGTTATGTTTGGTATA-3' using TaKaRa Ex Taq polymerase (Takara Biotech Inc.). These reactions generated a fusion product of approximately 6 kb called enu-3p::enu-3(+)::gfp. | ||
Used_for | Transgene_construct | WBTransgene00031024 | |
Reference | WBPaper00038105 |