WormBase Tree Display for Construct: WBCnstr00013794
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| WBCnstr00013794 | Other_name | Expr9204_Ex | |
|---|---|---|---|
| Summary | [pmaco-1::maco-1::gfp] | ||
| Driven_by_gene | WBGene00017066 | ||
| Gene | WBGene00017066 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [pmaco-1::maco-1::gfp] translational fusion. The cDNA sequence of maco-1 was obtained by sequencing clone yk1296a05 from the Kohara collection and by using RT- PCR. Primers specific for maco-1 exons and the SL1 spliced leader sequence were used to amplify maco-1 cDNA, and the PCR products sequenced. The maco-1 expression construct was generated using the Gateway system (Invitrogen). The Destination vector included 4 kb of the sequence upstream of the maco-1 start site but omitted 318 bp between the trans-splice site and the initiation codon. The Entry vector places maco-1 cDNA plus 9 bp of the sequence upstream in an artificial operon with gfp. | ||
| Clone | yk1296a05 | ||
| Used_for | Transgene_construct | WBTransgene00030974 | |
| Reference | WBPaper00038258 |
