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WormBase Tree Display for Construct: WBCnstr00012971

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Name Class

WBCnstr00012971Other_nameExpr7889_Ex
Summary[unc-41::unc-41-gfp]
Driven_by_geneWBGene00006777
GeneWBGene00006777
Fusion_reporterGFP
Type_of_constructTranscriptional_fusion
Construction_summary[unc-41::unc-41-gfp] transcriptional fusion. The GFP-UNC-41B WT (pMG13) C. elegans expression plasmid consisted of the 2.5-kb unc-41 promoter (from RM536), 0.85-kb GFP fragment (from fire lab vector 95.77), and 5.4-kb unc-41b cDNA plus 3'UTR (from RM 536) inserted into the EcoRI/SalI restriction sites of pGEM-3zf(+) using the following primers: 5'-AGGAGAATTCCTCCCGGCAATTCGTAATACGTC-3'; 5'-GGGTCCTGAAAATGTTCTATG-3'; 5'-ACATTCCCGGGATGGAACAAGCAGAAAAAGCA-3'; 5'-ACTTGTCGACCATGTGTCAGAGGTTTTCACCGTC-3'; 5'-AGATCCCGGGAGAACCTCCGCCTCCTTTGTATAGTTCATCCATGCCATG-3'; and 5'-ACCGCCCGGGATGAGTAAAGGAGAAGAACTTTTC-3'. --precise ends.
Used_forTransgene_constructWBTransgene00030176
ReferenceWBPaper00031369