WormBase Tree Display for Construct: WBCnstr00011699
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| WBCnstr00011699 | Other_name | Expr3858_Ex | |
|---|---|---|---|
| Summary | [ceh-20::ceh-20-cfp] | ||
| Driven_by_gene | WBGene00000443 | ||
| Gene | WBGene00000443 | ||
| Fusion_reporter | CFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [ceh-20::ceh-20-cfp] translational fusion. A full-length CEH-20 protein with a fusion of CFP to the C terminus of the protein. Introduction of this construct into ceh-20(ay42) mutants rescued the egg-laying defect. This construct was introduced into ced-3 mutants, in which P11.aaap and the other cells that undergo programmed cell death in the ventral nerve cord survive. To construct the pMP0017 Pceh-20::ceh-20-cfp translational fusion plasmid, a 3.6 kb SacI PflMI fragment of the ceh-20 genomic region, including ~1 kb of 5' sequence and 1 kb of 3' sequence was amplified by long PCR and cloned into pBSKSII+. Site-specific mutagenesis was used to change the stop codon to an As | ||
| Used_for | Transgene_construct | WBTransgene00028915 | |
| Reference | WBPaper00027049 |
