WormBase Tree Display for Construct: WBCnstr00011672
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| WBCnstr00011672 | Other_name | Expr3813_Ex | |
|---|---|---|---|
| Summary | [act-2::gfp] | ||
| Driven_by_gene | WBGene00000064 | ||
| Gene | WBGene00000064 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [act-2::gfp] translational fusion. PCR was used to amplify the act-2 promoter region (starting 2.9 kb 5' of the first codon) from the cosmid T04C12. The primers included a 5' Not1 site and a 3' Spe1 site for subcloning into pBluescript II KS+, using standard methods. Subsequently, both a PCR-amplified GFP fragment (with primer-encoded 5' and 3' Spe1 sites), and the act-2 exons and introns, plus 1 kb of 3' genomic sequence (with primer-encoded 5' Spe1 and a 3' Kpn1 sites) were subcloned into the promoter vector. A linker encoding seven amino acids (G, S, S, T, R, G, A) was used between the 3' end of the GFP and the start of ACT-2. | ||
| Used_for | Transgene_construct | WBTransgene00028888 | |
| Reference | WBPaper00027028 |
