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WormBase Tree Display for Construct: WBCnstr00011608

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Name Class

WBCnstr00011608Other_nameExpr3697_Ex
Summary[xrn-2::gfp-PEST]
Driven_by_geneWBGene00006964
Fusion_reporterGFP
Type_of_constructTranscriptional_fusion
Construction_summary[xrn-2::gfp-PEST] transcriptional fusion. For each gene, primers U1 and FL were used to amplify N2 genomic DNA corresponding to the initiation codon and upstream sequence, while primers FU and CAW31 (5$(B!l(B- GCCGCATAGTTAAGCCAGCC 3$(B!l(B) were used to amplify DNA corresponding to the gfp-pest or gfp gene and the 3$(B!l(B UTR of unc-54 from, respectively, pAF207 or pPD95_81. The PCR products were annealed and the resulting polynucleotide amplified using primers U2 and CAW32 (5$(B!l(B CCGCTTACAGACAAG CTGTGA 3$(B!l(B)
Used_forTransgene_constructWBTransgene00028825
ReferenceWBPaper00026763