WormBase Tree Display for Construct: WBCnstr00011215
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| WBCnstr00011215 | Other_name | Expr3202_Ex | |
|---|---|---|---|
| Summary | [F41E7.6::nls-gfp] | ||
| Driven_by_gene | WBGene00009622 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Transcriptional_fusion | ||
| Construction_summary | [F41E7.6::nls-gfp] translational and transcriptional fusion constructs. Genes to be tested were fused with the promoterless GFP vector pLS43. pLS43 is an adaptation of pPD95.67 with additional nuclear localization signals. Two PCR products were amplified for each gene. The first product was ~500 bases long and had HindIII and BamHI sites introduced by the amplifying oligonucleotides and overlapped the second exon of the gene to be analyzed with the GFP gene remaining in-frame. The 500-base amplified product was digested with HindIII and BamHI, then cloned into pLS43 digested with HindIII and BamHI. The second amplified product was longer, containing ~6 Kb (-6000 to -1) 5' of the start Met and extending 3' to overlap the first product by a minimum of 250 bases. | ||
| Clone | pPD95.67 | ||
| Used_for | Transgene_construct | WBTransgene00028435 | |
| Reference | WBPaper00024630 |
