WormBase Tree Display for Construct: WBCnstr00011031
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| WBCnstr00011031 | Other_name | Expr2941_Ex | |
|---|---|---|---|
| Summary | [vhp-1::gfp] | ||
| Driven_by_gene | WBGene00006923 | ||
| Gene | WBGene00006923 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [vhp-1::gfp] translational fusion. A SmaI-BalI 1.7 kbp genomic fragment, which contains the fifth to seventh exons of vhp-1, was obtained by PCR using the cosmid F08B1 as a template. This fragment was subcloned into the SmaI-BalI site of the GFP reporter vector pPD95.75. Then, a 16.7 kbp SmaI fragment from the cosmid K10C6, whose 5' end is 9.6 kbp upstream of the first exon and the 3' end SmaI site is within the fourth intron, was subcloned, yielding vhp-1gfp. --precise ends. | ||
| Clone | F08B1 | ||
| K10C6 | |||
| Used_for | Transgene_construct | WBTransgene00028257 | |
| Reference | WBPaper00024218 |
