WormBase Tree Display for Construct: WBCnstr00010943
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| WBCnstr00010943 | Other_name | Expr2774_Ex | |
|---|---|---|---|
| Summary | [sup-10::gfp] | ||
| Driven_by_gene | WBGene00006319 | ||
| Gene | WBGene00006319 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [sup-10::gfp] translational fusion containing 3.5 kb of sup-10 upstream sequence, the entire sup-10 coding region, and the gfp coding region fused just before the stop codon of sup-10. The sup-10::gfp fusion used in colocalization studies was constructed by subcloning a 7.3 kb MfeI genomic fragment from cosmid C27G6 containing sup-10 into the EcoRI site of pBSKII. A 6.4 kb PstI fragment was subcloned from this vector into p95.77, which contained 3.5 kb of promoter sequence and the sup-10 coding region. Through PCR techniques, a SalI site was introduced immediately preceding the stop codon of sup-10 to create an in-frame fusion with the gfp coding sequence. --precise ends. | ||
| Clone | C27G6 | ||
| Used_for | Transgene_construct | WBTransgene00028169 | |
| Reference | WBPaper00006135 |
