WormBase Tree Display for Construct: WBCnstr00010942
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| WBCnstr00010942 | Other_name | Expr2773_Ex | |
|---|---|---|---|
| Summary | [unc-93::gfp] | ||
| Driven_by_gene | WBGene00006822 | ||
| Gene | WBGene00006822 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [unc-93::gfp] translational fusion containing 5.3 kb of unc-93 upstream sequence, the entire unc-93 coding region, and the gfp coding region fused just before the stop codon of unc-93. The unc-93::gfp fusion was created by cloning a 12.9 kb SphIKpnI genomic fragment from cosmid C46F11 containing 5.3 kb of unc-93 upstream sequence, the entire coding sequence of unc-93 and 3.2 kb of unc-93 downstream region into the SphIKpnI sites of pPD95.79 creating pIP314. A KpnI site was introduced immediately preceding the unc-93 stop codon using PCR, and the KpnI fragment containing the unc-93 3 region was excised, creating plasmid pIP321 with a GFP fusion at the C terminus of UNC-93. --precise ends. | ||
| Clone | pPD95.79 | ||
| C46F11 | |||
| Used_for | Transgene_construct | WBTransgene00028168 | |
| Reference | WBPaper00006135 |
