WormBase Tree Display for Construct: WBCnstr00010864
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WBCnstr00010864 | Other_name | Expr2590_Ex | |
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Summary | [daf-12::gfp] | ||
Driven_by_gene | WBGene00000908 | ||
Fusion_reporter | GFP | ||
Construction_summary | [daf-12::gfp]. A 4 kb portion of the genomic sequence of that precedes daf-12 was amplified and it included the first two exons and first intron of the gene. The PCR product was amplified with oligonucleotides containing BamHI and XbaI restriction sites and these were used to clone into a TOPO TA vector and transformed into TOPO TFP cells. The promoter fragment was subcloned into GFP gene containing vector pPD95.73. | ||
Used_for | Transgene_construct | WBTransgene00028092 | |
Reference | WBPaper00004422 |