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WormBase Tree Display for Construct: WBCnstr00010864

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Name Class

WBCnstr00010864Other_nameExpr2590_Ex
Summary[daf-12::gfp]
Driven_by_geneWBGene00000908
Fusion_reporterGFP
Construction_summary[daf-12::gfp]. A 4 kb portion of the genomic sequence of that precedes daf-12 was amplified and it included the first two exons and first intron of the gene. The PCR product was amplified with oligonucleotides containing BamHI and XbaI restriction sites and these were used to clone into a TOPO TA vector and transformed into TOPO TFP cells. The promoter fragment was subcloned into GFP gene containing vector pPD95.73.
Used_forTransgene_constructWBTransgene00028092
ReferenceWBPaper00004422