WormBase Tree Display for Construct: WBCnstr00010733
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| WBCnstr00010733 | Other_name | Expr2258_Ex | |
|---|---|---|---|
| Summary | [wee-1.3::gfp] | ||
| Driven_by_gene | WBGene00006940 | ||
| Fusion_reporter | GFP | ||
| Construction_summary | [wee-1.3::gfp] tranlational fusion. High-fidelity PCR with primers TL49 (5' -ATGTATTAGCATGCTTCTTTAAACCCCAACCAT-3') and TL50 (5'CAACTCGAGCATGCCTGCGGAGTGACCAAAAG-3') allowed 1 kb of sequence 5' to the transcriptional start plus the first wee-1.3 exon to be amplified from N2 genomic DNA. These primers introduce SphI (TL49) and Kpn1 (TL50) sites into the resulting PCR product, which was restriction digested with these enzymes. This fragment was ligated into the SphI/KpnI-digested GFP-encoding plasmid pPD95.77 to create pSTL1. pSTL1 (10 ng/l) was microinjected together with pMH86 dpy-20+ rescuing plasmid (100 ng/l) into dpy-20(e1280ts). Injected animals were grown at 25 centigrades and transformed animals were identified as non-Dpy F1. Stable lines were used to determine the pattern of GFP fluorescence. --precise ends. | ||
| Clone | pMH86 | ||
| pPD95.77 | |||
| Used_for | Transgene_construct | WBTransgene00027964 | |
| Reference | WBPaper00005504 |
