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WormBase Tree Display for Construct: WBCnstr00010461

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Name Class

WBCnstr00010461Other_nameExpr1804_Ex
Summary[daf-9::gfp]
Driven_by_geneWBGene00000905
Fusion_reporterGFP
Construction_summary[daf-9::gfp] promoter fusion. Two gfp constructs were made, in which the 3 kb daf-9 promoter drives the expression of gfp with or without a nuclear localization signal. The 3 kb daf-9 promoter was amplified using 9P-5' (5'-CTCGCGCTTACTGCAGATTA-3') and 9P-3' (5' -CGGGATCCACTGTATTTTTTTCGTCGCCA-3'). The PCR fragment was digested and cloned into the PstI/BamHI sites of gfp vectors pPD95.67 (with nuclear localization signal) and pPD95.75 (without nuclear localization signal).
ClonepPD95.67
pPD95.75
Used_forTransgene_constructWBTransgene00027697
ReferenceWBPaper00005086