WormBase Tree Display for Construct: WBCnstr00010461
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WBCnstr00010461 | Other_name | Expr1804_Ex | |
---|---|---|---|
Summary | [daf-9::gfp] | ||
Driven_by_gene | WBGene00000905 | ||
Fusion_reporter | GFP | ||
Construction_summary | [daf-9::gfp] promoter fusion. Two gfp constructs were made, in which the 3 kb daf-9 promoter drives the expression of gfp with or without a nuclear localization signal. The 3 kb daf-9 promoter was amplified using 9P-5' (5'-CTCGCGCTTACTGCAGATTA-3') and 9P-3' (5' -CGGGATCCACTGTATTTTTTTCGTCGCCA-3'). The PCR fragment was digested and cloned into the PstI/BamHI sites of gfp vectors pPD95.67 (with nuclear localization signal) and pPD95.75 (without nuclear localization signal). | ||
Clone | pPD95.67 | ||
pPD95.75 | |||
Used_for | Transgene_construct | WBTransgene00027697 | |
Reference | WBPaper00005086 |