WormBase Tree Display for Construct: WBCnstr00010449
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WBCnstr00010449 | Other_name | Expr1793_Ex | |
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Summary | [C44H4.1::gfp] | ||
Driven_by_gene | WBGene00008093 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Transcriptional_fusion | ||
Construction_summary | [C44H4.1::gfp] transcriptional fusion. To construct GFP reporters, promoter fragments were amplified from wildtype (N2) genomic DNA using gene specific oligonucleotides. Each oligonucleotide pair was engineered with unique restriction sites to facilitate cloning of the promoter fragments into the GFP::HIS2B reporter vector pAP.10 (which was built from a GFP::HIS2B reporter kindly provided by G. Seydoux). This cloning strategy results in a transcriptional fusion to the GFP::HIS2B reporter. | ||
Used_for | Transgene_construct | WBTransgene00027685 | |
Reference | WBPaper00005056 |