WormBase Tree Display for Construct: WBCnstr00010262
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| WBCnstr00010262 | Other_name | Expr1403_Ex | |
|---|---|---|---|
| Summary | [flp-1::lacZ] | ||
| Driven_by_gene | WBGene00001444 | ||
| Gene | WBGene00001444 | ||
| Fusion_reporter | GFP | ||
| LacZ | |||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [flp-1::lacZ] translational fusion. Varying amounts of the flp-1 upstream region (107 bp, 232 bp, 332 bp, 1.5 kbp, and 6 kbp) were subcloned into lacZ (pPD21.28 and pPD16.01) or GFP (pPD95.75) reporter constructs and assayed for beta-galactosidase activity or fluorescence. One translational fusion construct in which 1.5 kbp of the flp-1 upstream region and part of the coding region was fused in-frame to the lacZ gene and the remaining part of the coding region was placed after the stop codon of the lacZ gene was also made. Reporter constructs were co-injected with the rol-6 pRF4 plasmid. All transgenic lines showed flp-1 expression only in anterior head neurons, and for the most part, all transgenic lines had the same expression pattern. | ||
| Clone | pRF4 | ||
| pPD95.75 | |||
| pPD21.28 | |||
| Used_for | Transgene_construct | WBTransgene00027505 | |
| Reference | WBPaper00003193 |
