WormBase Tree Display for Construct: WBCnstr00010220
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| WBCnstr00010220 | Other_name | Expr1327_Ex | |
|---|---|---|---|
| Summary | [lir-1::lacZ] | ||
| Driven_by_gene | WBGene00003044 | ||
| Fusion_reporter | LacZ | ||
| Construction_summary | [lir-1::lacZ]. Plasmid pML169 carries the F18A1 BamHI/EcoRI fragment spanning nts 12,01327,160 (an 8-mer BglII linker was added at the EcoRI site after making it blunt), the BamHI/StuI fragment containing the beta-galactosidase coding sequence from plasmid pPD16.43, and, finally, a fragment obtained after ExoIII nuclease digestion that starts at the EcoRI site at position 27,504 (transformed into a BglII site with a linker) and ends at position 28,254. Plasmid pML165 has a structure similar to pML169, except that it starts at the EagI site at position 17,709, and the lacZ is inserted at the NdeI site at position 27,501 of F18A1 instead of the EcoRI site at position 27,160. | ||
| Clone | F18A1 | ||
| pPD16.43 | |||
| Used_for | Transgene_construct | WBTransgene00027465 | |
| Reference | WBPaper00003532 |
