WormBase Tree Display for Construct: WBCnstr00009415
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| WBCnstr00009415 | Summary | [Pmec-12::MEC-12(K40R)::3'UTR mec-12] | |
|---|---|---|---|
| Driven_by_gene | WBGene00003175 | ||
| Gene | WBGene00003175 | ||
| UTR_3 | WBGene00003175 | ||
| Construction_summary | A MEC-12 plasmid, pMEC-12, was constructed using a MEC-12 cDNA and genomic sequence. Using overlapping PCR, the K40 codon was mutated to an R codon on pMEC-12 along with silent substitutions creating a restriction site (PvuI site for R40 plasmid). | ||
| Used_for | Transgene_construct | WBTransgene00009749 | |
| Reference | WBPaper00037127 | ||
| WBPaper00044649 |
