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WormBase Tree Display for Construct: WBCnstr00009414

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Name Class

WBCnstr00009414Summary[Pmec-12::MEC-12(K40Q)::3'UTR mec-12]
Driven_by_geneWBGene00003175
GeneWBGene00003175
UTR_3WBGene00003175
Construction_summaryA MEC-12 plasmid, pMEC-12, was constructed using a MEC-12 cDNA and genomic sequence. Using overlapping PCR, the K40 codon was mutated to a Q codon on pMEC-12 along with silent substitutions creating a restriction site (HindIII site for Q40 plasmid).
Used_forTransgene_constructWBTransgene00009748
ReferenceWBPaper00037127