Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for Construct: WBCnstr00009220

expand all nodes | collapse all nodes | view schema

Name Class

WBCnstr00009220Summary[Punc-86::TRIM-9(C22S, H24Y); Podr-1::RFP]
Driven_by_geneWBGene00006818
GeneWBGene00016539
Construction_summaryFull-length trim-9 cDNA was amplified from the cDNA clone yk801c07 and inserted into the delta-PSM vector between the Nhe I and EcoR V sites. The 5.1 kb unc-86 promoter from Punc-86::mCherry was inserted upstream of the trim-9 cDNA for tissue specific rescue. Punc-86::TRIM-9(C22S, H24Y) was generated by site direct mutagenesis with primers containing the mutant sequences.
Used_forTransgene_constructWBTransgene00009545
ReferenceWBPaper00038152