WormBase Tree Display for Construct: WBCnstr00009220
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WBCnstr00009220 | Summary | [Punc-86::TRIM-9(C22S, H24Y); Podr-1::RFP] | |
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Driven_by_gene | WBGene00006818 | ||
Gene | WBGene00016539 | ||
Construction_summary | Full-length trim-9 cDNA was amplified from the cDNA clone yk801c07 and inserted into the delta-PSM vector between the Nhe I and EcoR V sites. The 5.1 kb unc-86 promoter from Punc-86::mCherry was inserted upstream of the trim-9 cDNA for tissue specific rescue. Punc-86::TRIM-9(C22S, H24Y) was generated by site direct mutagenesis with primers containing the mutant sequences. | ||
Used_for | Transgene_construct | WBTransgene00009545 | |
Reference | WBPaper00038152 |