WormBase Tree Display for Construct: WBCnstr00008915
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WBCnstr00008915 | Summary | [DAF-36] | |
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Gene | WBGene00007536 | ||
Construction_summary | The ORF of C. elegans daf-36 was amplified by PCR using the Expanded High Fidelity PCR enzyme (Roche) with reverse-transcribed templates from wild type N2 larvae and the following primers: 5'-GGATCCATGCTACTGGAACAGATTTGGG-3' and 5'-GCGGCCGCGCCAGTCAAAAATTGATTTTGCTTGATTG-3' with BamHI and NotI sites. The PCR product was subcloned into pCR2.1-TOPO (Invitrogen). The subcloned pCR2.1 plasmid was digested with BamHI-NotI. The digested fragment was ligated with BglII/NotI-digested HA-pUAST, leading to the daf-36-HA-pUAST plasmid. | ||
Used_for | Transgene_construct | WBTransgene00009229 | |
Reference | WBPaper00038482 |