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WormBase Tree Display for Construct: WBCnstr00008915

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Name Class

WBCnstr00008915Summary[DAF-36]
GeneWBGene00007536
Construction_summaryThe ORF of C. elegans daf-36 was amplified by PCR using the Expanded High Fidelity PCR enzyme (Roche) with reverse-transcribed templates from wild type N2 larvae and the following primers: 5'-GGATCCATGCTACTGGAACAGATTTGGG-3' and 5'-GCGGCCGCGCCAGTCAAAAATTGATTTTGCTTGATTG-3' with BamHI and NotI sites. The PCR product was subcloned into pCR2.1-TOPO (Invitrogen). The subcloned pCR2.1 plasmid was digested with BamHI-NotI. The digested fragment was ligated with BglII/NotI-digested HA-pUAST, leading to the daf-36-HA-pUAST plasmid.
Used_forTransgene_constructWBTransgene00009229
ReferenceWBPaper00038482