WormBase Tree Display for Construct: WBCnstr00008857
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| WBCnstr00008857 | Other_name | [CEH-23::GFP] | |
|---|---|---|---|
| Expr9598_Ex | |||
| Summary | [gfp::ceh-23(+); mec-7::rfp] | ||
| Gene | WBGene00000446 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | translational fusion. A 10.3 Kbp genomic fragment containing the 7.3 Kbp sequence upstream of the ceh-23 coding region, the 1.8 Kbp ceh-23 coding region, and the 1.1 Kbp sequence downstream of the ceh-23 coding region was amplified from genomic DNA by PCR. A GFP coding sequence without stop codon was amplified by PCR from the plasmid pPD95-81. The amplified GFP sequence was then fused in frame at the N-terminal of the ceh-23 coding region into the 10.3 Kbp genomic fragment described above. --precise ends. | ||
| Used_for | Transgene_construct | WBTransgene00009170 | |
| Reference | WBPaper00039825 |
