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WormBase Tree Display for Construct: WBCnstr00008438

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Name Class

WBCnstr00008438Summary[enu-3p::gfp; myo-2p::yfp]
Driven_by_geneWBGene00010362
Fusion_reporterGFP
Type_of_constructTranscriptional_fusion
Construction_summaryA GFP gene with an unc-54 3' UTR PCR product was made from pPD95.75 using primers; 5'-AGCTTGCATGCCTGCAGGTCG-3' and 5'-AAGGGCCCGTACGGCCGACTA-3' (Fire et al., 1994; www.addgene.org). The two PCR products were then fused using nested primers: the primer used to generate 3 kb of the H04D03.1 promoter was; 5'-AGTCGACCTGCAGGCATGCAAGCTGAGTAGGGGCAGGGACTAT-3', at the transcriptional initiator located 101 bases 5' to the initiator methionine. The PCR product was injected with myo-2p::yfp as a co-transformation marker to give rqEx2.
Used_forTransgene_constructWBTransgene00008738
ReferenceWBPaper00038105