WormBase Tree Display for Construct: WBCnstr00008438
expand all nodes | collapse all nodes | view schema
WBCnstr00008438 | Summary | [enu-3p::gfp; myo-2p::yfp] | |
---|---|---|---|
Driven_by_gene | WBGene00010362 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Transcriptional_fusion | ||
Construction_summary | A GFP gene with an unc-54 3' UTR PCR product was made from pPD95.75 using primers; 5'-AGCTTGCATGCCTGCAGGTCG-3' and 5'-AAGGGCCCGTACGGCCGACTA-3' (Fire et al., 1994; www.addgene.org). The two PCR products were then fused using nested primers: the primer used to generate 3 kb of the H04D03.1 promoter was; 5'-AGTCGACCTGCAGGCATGCAAGCTGAGTAGGGGCAGGGACTAT-3', at the transcriptional initiator located 101 bases 5' to the initiator methionine. The PCR product was injected with myo-2p::yfp as a co-transformation marker to give rqEx2. | ||
Used_for | Transgene_construct | WBTransgene00008738 | |
Reference | WBPaper00038105 |