WormBase Tree Display for Construct: WBCnstr00008437
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| WBCnstr00008437 | Summary | [enu-3p::enu-3(+)::gfp; myo-2p::yfp] | |
|---|---|---|---|
| Driven_by_gene | WBGene00010362 | ||
| Gene | WBGene00010362 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | The enu-3 product was made using the outside H04D03.1 primer corresponding to 3 kb upstream of the initiator ATG; 5'-CGTGTAACTCCTTTCATCTTGGCAACCATAGCTCCG-3' and the fusion primer located right before the stop codon of H04D03.1; 5'-AGTCGACCTGCAGGCATGCAAGCTATCGCGTGGCTTGTCCACAGT-3' using LongAmp Taq Polymerase. A GFP gene with an unc-54 3' UTR PCR product was made from pPD95.75 using primers; 5'-AGCTTGCATGCCTGCAGGTCG-3' and 5'-AAGGGCCCGTACGGCCGACTA-3' (Fire et al., 1994; www.addgene.org). The two PCR products were then fused using nested primers: for H04D03.1; 5'-CTGTTGAAGGAGATCCTCTTT-3' and for GFP; 5'-GGAAACAGTTATGTTTGGTATA-3' using TaKaRa Ex Taq polymerase (Takara Biotech Inc.). These reactions generated a fusion product of approximately 6 kb and this was used for microinjection at approximately 20 ng/ul with the coinjection marker pPD132.102 (myo-2p::yfp) into him-5(e1490);dpy-20(e1282) hermaphrodites. | ||
| Used_for | Transgene_construct | WBTransgene00008737 | |
| Reference | WBPaper00038105 |
