WormBase Tree Display for Construct: WBCnstr00007634
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| WBCnstr00007634 | Summary | [Pmadd-2::MADD-2::GFP; Podr-1::RFP] | |
|---|---|---|---|
| Driven_by_gene | WBGene00016539 | ||
| Gene | WBGene00016539 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | This madd-2::gfp fusion was constructed by cloning a PCR fragment containing the entire madd-2 genomic sequence without the initiator methionine in frame into the XmaI site of the GFP expression vector pPD95.77. A PCR fragment containing >700 nucleotides of overlapping madd-2 genomic sequence, including the start codon, and over 14 kb of upstream sequence, was amplified from the YAC Y75B7. Both the madd-2::gfp genomic fusion gene and the overlapping PCR product were injected into N2 animals at 5, 30, and 100 ng/ul along with odr-1::rfp at 50 ng/ul as a coinjection marker, (Hao et al., 2010). | ||
| Used_for | Transgene_construct | WBTransgene00007882 | |
| Reference | WBPaper00036484 |
