WormBase Tree Display for Construct: WBCnstr00000416
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WBCnstr00000416 | Public_name | pCL25 |
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Other_name | Expr10722_Ex | |
[hsp-16.2::gfp; rol-6(su1006)] | ||
Summary | [hsp-16.2p::GFP] | |
Driven_by_gene | WBGene00002016 | |
Fusion_reporter | GFP | |
Type_of_construct | Translational_fusion | |
Construction_summary | The 341 bp hsp-16.2 promoter region was recovered as a HindIII/BamHI fragment from expression vector pPD49.78, and ligated between the unique HindIII/BamHI sites of pGFP-TT. The pGFP-TT vector was derived from pTU61 by site-directed mutagenesis, and contains Ser65Thr and IIe167Thr mutations that increase the fluorescence of GFP. | |
Used_for (2) | ||
Reference (46) |