WormBase Tree Display for Antibody: WBAntibody00000986
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| WBAntibody00000986 | Summary | Rabbit polyclonal antibody against full-length MEI-2 recombinant protein. | ||
|---|---|---|---|---|
| Public_name | [WBPaper00004129]:mei-2 | |||
| Other_name | MS-3F | |||
| Gene | WBGene00003184 | |||
| Isolation | Original_publication | WBPaper00004129 | ||
| Location | HR | |||
| Clonality | Polyclonal | |||
| Antigen | Protein | Full-length mei-2 cDNA was obtained by RTPCR amplification using 5 and 3 primers containing linkers for insertion into a HIS-tagged fusion vector (QE30) from the QiaExpression kit (Qiagen). Bacterially expressed protein was purified under denaturing conditions (8M urea) by passage through a NiNT Agarose column, using manufacturer's protocol. This purified protein was electrophoresed through an SDS-PAGE gel and excised to immunize rabbits. Antiserum (MS-3F) was affinity purified as follows: an amino-terminal portion (residues 299) of MEI-2 was fused to GST (Promega), bacterially expressed, and purified under native conditions. Approximately 2 mg of purified GST-MEI-2(299) protein was coupled to cyanogen bromide-activated Sepharose (Pharmacia). | ||
| Animal | Rabbit | |||
| Interactor | WBInteraction000556053 | |||
| WBInteraction000556054 | ||||
| WBInteraction000556055 | ||||
| WBInteraction000556056 | ||||
| Reference | WBPaper00004129 | |||
| WBPaper00006085 | ||||
| WBPaper00025044 | ||||
| WBPaper00049964 |
