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WormBase Tree Display for Antibody: WBAntibody00000885

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Name Class

WBAntibody00000885SummaryRat polyclonal Anti-SMA-9 protein antibodies were generated against the 70 amino acids that are unique to the abundantly expressed class II isoforms.
Public_name[pmid14627718]:sma-9
Other_nameCUMC-R2 TB2
GeneWBGene00004862
IsolationLocationCS
LW
ClonalityPolyclonal
AntigenProteinPlasmid pJKL547.1, which contains the cDNA fragment corresponding to the 70 amino acids cloned into pGEX-2T, was transformed into BL21 cells. Fusion proteins were first purified using Glutathione sepharose 4B beads (Amersham Biosciences) and further purified by SDS-PAGE. Gel slices containing the purified fusion proteins were used to immunize rats by Cocalico Biologicals, PA.
AnimalRat
Expr_patternExpr2783
ReferenceWBPaper00006346
WBPaper00027702