WormBase Tree Display for Antibody: WBAntibody00000629
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| WBAntibody00000629 | Summary | Polyclonal rabbit antibody against CUL-3 recombinant protein. | ||
|---|---|---|---|---|
| Public_name | [cgc5914]:cul-3 | |||
| Gene | WBGene00000838 | |||
| Isolation | Original_publication | WBPaper00005914 | ||
| Location | EU | |||
| Clonality | Polyclonal | |||
| Antigen | Protein | The vector pGEX-4T (Pharmacia) was used to express GST fusions of CUL-3 in E. coli BL21. Inclusion bodies were prepared and loaded onto SDS-PAGE according to standard procedures. Fusion protein was electroeluted and was used to immunize rabbits. Antibodies were affinity purified on immobilized GST-CUL-3 fusion protein. | ||
| Animal | Rabbit | |||
| Expr_pattern | Expr2600 | |||
| Reference | WBPaper00005914 | |||
| WBPaper00006054 | ||||
| WBPaper00033469 | ||||
| WBPaper00029237 |
