WormBase Tree Display for Antibody: WBAntibody00000367
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| WBAntibody00000367 | Summary | Rabbit polyclonal antibodies were generated to IMA protein and affinity purified. | ||
|---|---|---|---|---|
| Public_name | [cgc4711]:ima-1 | |||
| Gene | WBGene00002072 | |||
| Isolation | Clonality | Polyclonal | ||
| Antigen | Protein | PCR products encoding amino acids 490-524 of IMA-1 were inserted in frame with the glutathione-S-transferase (GST) protein of the pGEX-4T-1 vector (Amersham Pharmacia Biotech, Picataway, NJ). Recombinant protein induction and purification were performed as described in the manufacturer's instructions (Amersham Pharmacia). Antibodies raised against GST-IMA-1 were purified by adsorption of antibodies to GST on a GST-Affi-Gel column (BioRad Laboratories, Hercules, CA) followed by binding and elution of anti-IMA-1 antibodies from a GST-IMA-1 Affi-Gel column. | ||
| Animal | Rabbit | |||
| Expr_pattern | Expr1339 | |||
| Reference | WBPaper00004711 |
