WormBase Tree Display for Antibody: WBAntibody00000327
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| WBAntibody00000327 | Summary | Polyclonal rabbit antibody against recombinant protein. Anti-NID-1 antiserum that reacts with all isoforms. | ||
|---|---|---|---|---|
| Public_name | [cgc4414]::anti-NID-1 | |||
| Gene | WBGene00003738 | |||
| Isolation | Location | CH | ||
| GS | ||||
| Clonality | Polyclonal | |||
| Antigen | Protein | A GST-NID-1 fusion construct was generated by cloning the 1.3-kb NciI-XhoI (nucleotides 7820 to 9521 genomic) fragment from the cDNA yk38f9 (D36924, D33953) into the pGEX-4T1 (Pharmacia Biotechnology, Piscataway, NJ) vector. This construct expresses the last 22 amino acids of the rod domain and the complete G3 domain of NID-1 fused to the carboxyl end of GST. The fusion protein was SDS-PAGE purified and used to immunize rabbits. An MBP-NID-1(G3 domain) fusion protein was generated by inserting the 1.1-kb PvuII-XhoI fragment (nucleotides 8239 to 9521) from the yk38f9 cDNA into the pMAL-c2 vector (New England Biolabs, Beverly, MA). The purified pMAL fusion protein was used to affinity purify the rabbit antisera. | ||
| Animal | Rabbit | |||
| Expr_pattern | Expr1074 | |||
| Expr2567 | ||||
| Reference | WBPaper00024330 | |||
| WBPaper00027321 | ||||
| WBPaper00005871 | ||||
| WBPaper00004414 | ||||
| WBPaper00042427 |
