WormBase Tree Display for Antibody: WBAntibody00000262
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WBAntibody00000262 | Summary | Polyclonal rabbit antibody against recombinant protein. | ||
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Public_name | [cgc3869]:rhi-1 | |||
Gene | WBGene00004356 | |||
Isolation | Clonality | Polyclonal | ||
Antigen | Protein | The coding region of the CeRhoGDI cDNA clone was isolated by PCR using specific primers (forward primer: 5H-ATCCCGGGATGCTCGACCACGAGAA-3H and reverse primer: 5H-AGCCCGGGCTACTCCTTGGTAATGTG-3H), and subcloned into the bacterial glutathione S-transferase (GST)- and maltose binding protein (MBP)-expression vectors, pGEX-3 (Pharmacia Biotech Inc.) and pMAL-c2 (New England Biolabs), respectively. GST/CeRhoGDI and MBP/CeRhoGDI fusion proteins were induced and proteins purified. | ||
Animal | Rabbit | |||
Expr_pattern | Expr97 | |||
Reference | WBPaper00003869 |