WormBase Tree Display for Antibody: WBAntibody00000234
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| WBAntibody00000234 | Summary | Polyclonal rabbit antibody against SYN-4 recombinant protein. | ||
|---|---|---|---|---|
| Public_name | [cgc3608]::anti-SYN-4 | |||
| Gene | WBGene00006374 | |||
| Isolation | Original_publication | WBPaper00003608 | ||
| Location | BS | |||
| MG | ||||
| Clonality | Polyclonal | |||
| Antigen | Protein | cDNA clones yk175g4 and yk215b11 were sequenced and the structure of the gene as predicted by the GENEFINDER program was confirmed. Syn-4 was expressed as a GST fusion protein (a 350 bp fragment derived from exon 4 was cloned into pGEX-4T). Upon induction in Escherichia coli, the fusion protein accumulated in inclusion bodies. The fusion protein was extracted from the insoluble material using 8 M urea, purified under denaturing conditions on a Hi-trap Q sepharose column (Pharmacia), and renatured by dialysis. | ||
| Animal | Rabbit | |||
| Expr_pattern | Expr2735 | |||
| Expr8957 | ||||
| Reference | WBPaper00005155 | |||
| WBPaper00004265 | ||||
| WBPaper00065052 | ||||
| WBPaper00031318 | ||||
| WBPaper00003608 | ||||
| WBPaper00032152 | ||||
| WBPaper00005100 | ||||
| WBPaper00044292 | ||||
| WBPaper00051434 |
