WormBase Tree Display for Antibody: WBAntibody00000175
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| WBAntibody00000175 | Summary | Affinity-purified rabbit polyclonal PHA-4 antibody against recombinant protein. | ||
|---|---|---|---|---|
| Public_name | [cgc3068]:pha-4 | |||
| Gene | WBGene00004013 | |||
| Isolation | Original_publication | WBPaper00003068 | ||
| Location | JM | |||
| Clonality | Polyclonal | |||
| Antigen | Protein | A 175-base-pair BamHI-HincII fragment from pha-4 cDNA, encoding 58 amino acids located towards the C terminus end, outside of the DNA binding domain, was cloned in-frame into the BamHI-SmaI sites of pGEX-1 (Pharmacia Biotechnology) as well as into the SmaI site of pTRXfus (Invitrogen). A GST/PHA-4 fusion protein was induced and purified and used to immunize rabbits. | ||
| Animal | Rabbit | |||
| Expr_pattern | Expr1431 | |||
| Reference | WBPaper00005395 | |||
| WBPaper00003068 |
