WormBase Tree Display for Expr_pattern: Expr9948
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| Expr9948 | Expression_of | Gene | WBGene00002282 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00002282 | |||
| Expression_data | Life_stage | WBls:0000003 | ||
| WBls:0000041 | ||||
| WBls:0000023 | ||||
| Anatomy_term | WBbt:0004540 | Certain | ||
| WBbt:0005733 | Certain | |||
| WBbt:0005778 | Certain | |||
| WBbt:0005812 | Certain | |||
| WBbt:0006748 | Certain | |||
| WBbt:0008182 | Certain | |||
| GO_term | GO:0045177 | |||
| Subcellular_localization | In almost all epithelia where it was expressed, LET-4::GFP appeared strongly apically enriched. In the excretory duct and pore, LET-4::GFP lined the luminal membrane. In the epidermis, it was distributed across the apical surfaces of most dorsal and ventral epidermal cells but was observed more weakly or variably in the lateral (seam) epidermis. The fusion was not strongly enriched at apical junctions based on co-visualization with DLG-1/Discs Large::mcherry. The fusion protein partially overlapped with but did not strongly colocalize with transepidermal intermediate filaments at hemidesmosomes. The fusion was present in many large puncta, potentially representing a vesicular compartment trafficking to or from the membrane. Interestingly, the one exception to the apical localization of LET- 4::GFP was the excretory canal cell. At the 1.5-fold stage, when LET-4::GFP was transiently expressed in the canal cell, LET- 4::GFP localized uniformly around the plasma membrane and not to the developing internal lumen. | |||
| Type | Reporter_gene | |||
| Pattern | LET-4::GFP was expressed in a subset of epithelial cells, including epidermal, vulval and rectal cells and the excretory duct and pore. Expression began around the ventral enclosure stage of embryogenesis and continued through larval development, but then decreased in adulthood. Expression was absent from internal epithelia such as the gut and pharyngeal tubes. LET-4::GFP was transiently expressed in the excretory canal cell at the 1.5-fold stage, but no longer visible in this cell by hatch. Notably, with the exception of the canal cell, the epithelia that expressed LET-4 were those that would eventually become cuticle-lined. | |||
| Reference | WBPaper00040698 | |||
| Transgene | WBTransgene00015369 |
