WormBase Tree Display for Expr_pattern: Expr973
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| Expr973 | Expression_of | Gene | WBGene00003106 | ||
|---|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003106 | ||||
| Expression_data | Life_stage | WBls:0000022 | |||
| WBls:0000018 | |||||
| WBls:0000019 | |||||
| WBls:0000021 | |||||
| Anatomy_term | WBbt:0003825 | Certain | |||
| WBbt:0004092 | Certain | ||||
| WBbt:0004094 | Certain | ||||
| WBbt:0004799 | Certain | ||||
| WBbt:0005135 | Partial | ||||
| Remark | Expressed in one neuronal cell nucleus in head ganglion. | ||||
| WBbt:0005734 | Partial | ||||
| Remark | Observed in two posterior nuclei of hyp7 | ||||
| WBbt:0005829 | Uncertain | ||||
| Remark | Transient expression and not reproducible. | ||||
| WBbt:0006795 | Partial | ||||
| WBbt:0006985 | Certain | ||||
| WBbt:0006986 | Certain | ||||
| GO_term | GO:0005634 | ||||
| Subcellular_localization | mab-9 localizes to the nucleus. | ||||
| Type | Reporter_gene | ||||
| Pattern | mab-9 is expressed in very few cells throughout development. In L1 larvae, mab-9::GFP expression is seen in the posterior hindgut cells B and F, the two cells that take on inappropriate lineage fates in mab-9 mutants. Expression is also seen in two posterior hypodermal nuclei of the large syncytial cell hyp7 and in the nervous system, where mab-9::GFP expression is evident in two neuronal nuclei of the lumbar ganglion. These are most likely to be the two nuclei of the interneuron PVC. In addition, mab-9::GFP expression is always observed in a single neuronal nucleus of the head ganglion. The neuronal and hypodermal expression pattern of mab-9 is the same in males and hermaphrodites. mab-9::GFP expression is observed in both B.a and B.p. In late larval males, the expression pattern of mab-9 is more widespread in nuclei of the proctodeum. In adult hermaphrodites, mab-9::GFP expression is maintained in the same six cells that expressed mab-9 during larval development. A construct in which a 5.2-kb mab-9 promoter-only cassette was cloned in front of GFP (vector pPD96.04) was found to have a very similar expression pattern when present in wild-type worms as an extrachromosomal array. mab-9 is also expressed during embryogenesis. mab-9::GFP expression is first seen at the 1.5-fold stage, 430 min after first cleavage, in three to four nuclei around the presumptive rectum [B, F, and hyp7]. In 3-fold embryos, mab-9::GFP expression becomes very intense in the nuclei of B and F, and weaker expression can also be observed in nuclei of the ventral cord. This ventral cord expression appears to be transient and is not observed reproducibly as larval development ensues. | ||||
| Picture | WBPicture0000008393 | ||||
| Reference | WBPaper00003958 | ||||
| Transgene | WBTransgene00000659 |
