WormBase Tree Display for Expr_pattern: Expr9598
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| Expr9598 | Expression_of | Gene | WBGene00000446 | ||
|---|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000446 | ||||
| Expression_data | Anatomy_term | WBbt:0003679 | Partial | ||
| Remark | Possibly expressed in the pair of CAN neurons in the central body and 12 sets of sensory neurons in the head (10 pairs) and the tail (2 pairs). | ||||
| WBbt:0005772 | Uncertain | ||||
| Remark | The intestinal expression was only detected in 50% of the transgenic worms. | ||||
| GO_term | GO:0005634 | ||||
| Subcellular_localization | Enriched nuclear localization of CEH-23 was detected in both the neurons and the intestine. | ||||
| Type | Reporter_gene | [CEH-23::GFP] translational fusion. A 10.3 Kbp genomic fragment containing the 7.3 Kbp sequence upstream of the ceh-23 coding region, the 1.8 Kbp ceh-23 coding region, and the 1.1 Kbp sequence downstream of the ceh-23 coding region was amplified from genomic DNA by PCR. A GFP coding sequence without stop codon was amplified by PCR from the plasmid pPD95-81. The amplified GFP sequence was then fused in frame at the N-terminal of the ceh-23 coding region into the 10.3 Kbp genomic fragment described above. --precise ends. | |||
| Pattern | The expression of CEH-23::GFP was restricted to a handful of neurons and the intestine of the worm. Although the neurons in which CEH-23 is expressed have not been identified, the observed neuronal expression of the CEH-23::GFP construct resembles that of a previously published GFP construct fused to a partial CEH-23 (gmIs18[ceh-23::gfp]), which was reported to express in the pair of CAN neurons in the central body and 12 sets of sensory neurons in the head (10 pairs) and the tail (2 pairs). While the neuronal expression of CEH-23 was found in all the transgenic worms, the intestinal expression was only detected in ,50% of the transgenic worms with a stronger expression in the posterior intestine. | ||||
| The expression of CEH-23::GFP was restricted to a handful of neurons and the intestine of the worm. Although the neurons in which CEH-23 is expressed have not been identified, the observed neuronal expression of the CEH-23::GFP construct resembles that of a previously published GFP construct fused to a partial CEH-23 (gmIs18[ceh-23::gfp]), which was reported to express in the pair of CAN neurons in the central body and 12 sets of sensory neurons in the head (10 pairs) and the tail (2 pairs). While the neuronal expression of CEH-23 was found in all the transgenic worms, the intestinal expression was only detected in 50% of the transgenic worms with a stronger expression in the posterior intestine. | |||||
| Picture | WBPicture0000012548 | ||||
| Reference | WBPaper00039825 | ||||
| Transgene | WBTransgene00009170 |
