WormBase Tree Display for Expr_pattern: Expr957

Expr957 Expression_of: Gene: WBGene00001653
    Reflects_endogenous_expression_of: WBGene00001653
  Homol: Homol_homol: K04D7:Expr
  Expression_data: Anatomy_term: WBbt:0004448 Uncertain
        Remark: Expressed in four cells in developing vulva.
      WBbt:0004477 Uncertain
        Remark: Expressed in four cells in developing vulva.
      WBbt:0004574 Uncertain
        Remark: Expressed in four cells in developing vulva.
      WBbt:0004577 Uncertain
        Remark: Expressed in four cells in developing vulva.
      WBbt:0005175 Partial
      WBbt:0005784 Certain
      WBbt:0006983 Uncertain
        Remark: Expressed in four cells in developing vulva.
      WBbt:0006984 Uncertain
        Remark: Expressed in four cells in developing vulva.
      WBbt:0006987 Uncertain
        Remark: Expressed in four cells in developing vulva.
      WBbt:0006988 Uncertain
        Remark: Expressed in four cells in developing vulva.
      WBbt:0006989 Uncertain
        Remark: Expressed in four cells in developing vulva.
      WBbt:0006990 Uncertain
        Remark: Expressed in four cells in developing vulva.
      WBbt:0006991 Uncertain
        Remark: Expressed in four cells in developing vulva.
      WBbt:0007252 Uncertain
        Remark: Expressed in four cells in developing vulva.
      WBbt:0007253 Uncertain
        Remark: Expressed in four cells in developing vulva.
      WBbt:0007254 Uncertain
        Remark: Expressed in four cells in developing vulva.
      WBbt:0007255 Uncertain
        Remark: Expressed in four cells in developing vulva.
      WBbt:0007256 Uncertain
        Remark: Expressed in four cells in developing vulva.
      WBbt:0007260 Uncertain
        Remark: Expressed in four cells in developing vulva.
      WBbt:0007264 Uncertain
        Remark: Expressed in four cells in developing vulva.
      WBbt:0007265 Uncertain
        Remark: Expressed in four cells in developing vulva.
      WBbt:0007266 Uncertain
        Remark: Expressed in four cells in developing vulva.
    GO_term: GO:0005634
      GO:0043073
  Subcellular_localization: nucleus
  Type: Reporter_gene
  Pattern: GON-4::GFP was found in the nucleus. Furthermore, GON-4::GFP was found in the somatic gonadal precursor cells, Z1 and Z4, and their descendants from L1 until the early L3 stage. Expression was weak, but reproducible. During L4, GFP was observed in four nuclei of developing vulva, but not in other nongonadal cells. Intriguingly, GON-4::GFP was detected in a scattering of germ-line nuclei of rescued animals. In contrast to the somatic gonadal expression, which occurred during early gonadogenesis, germ-line expression started in the second half of L4 and continued in adulthood.
  Remark: This gon-4::GFP translational fusion rescues gon-4(q519) mutants to fertility when incorporated into the extrachromosomal array qEx453. Nine other rescuing lines also exhibited the same pattern. Preliminary results with polyclonal antibodies raised to the C-terminal portion of GON-4 are consistent with expression of GON-4 in the nuclei of dissected germ lines in mid- to late L4s and adults. These same antibodies have not proven useful with whole mounts of early larvae, which require a different protocol for antibody staining. Expression of the gon-4::GFP transgene was silenced in both somatic gonad and germ line.
  Reference: WBPaper00004450
  Transgene: WBTransgene00031002