WormBase Tree Display for Expr_pattern: Expr9090
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| Expr9090 | Expression_of | Gene | WBGene00016539 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00016539 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| WBls:0000015 | ||||
| WBls:0000021 | ||||
| Anatomy_term | WBbt:0003675 | Partial | ||
| WBbt:0003679 | Partial | |||
| WBbt:0004757 | Certain | |||
| WBbt:0004758 | Certain | |||
| WBbt:0005733 | Certain | |||
| GO_term | GO:0005737 | |||
| Subcellular_localization | In most cells, MADD-2::GFP protein was in irregular puncta in the cytoplasm. | |||
| Type | Reporter_gene | |||
| Pattern | A rescuing madd-2::gfp transgene was expressed in most neurons, in hypodermis, and in muscle cells. In many neurons, madd-2::gfp expression was transient, peaking during periods of axon outgrowth and guidance. madd-2::gfp was most widely and strongly expressed in the embryo, particularly during the initial period of axon outgrowth at 350 to 400 min of development. At this stage, madd-2::gfp was prominent in the anterior embryo, including the developing neurons of the nerve ring, in the developing motor neurons of the ventral nerve cord, in posterior neurons, and in hypodermal cells. At ~430 min of development (1.5-fold stage), the madd-2::gfp transgene exhibited a more restricted pattern of expression in the anterior, ventral, and posterior regions of the embryo. The extinction of reporter gene expression in many neurons coincides with the period when most axons in the nerve ring and ventral cord have reached their targets. At the first larval stage, continued high expression of the madd-2::gfp transgene was observed in some head neurons, head muscles, ventral motor neurons, neurons in the tail, and hypodermal cells. As the animals developed from larvae into adults, madd-2::gfp expression decreased substantially in head and tail neurons. madd-2::gfp expression was detected in the HSN motor neurons around the third larval stage, during the period of its ventral axon outgrowth. Although reporter gene fusions should be interpreted with caution, these results suggest that MADD-2 could be present in many Netrin-responsive neurons at the time of axon guidance. | |||
| Picture | WBPicture0000010062 | |||
| Remark | Picture: Figure 4. | |||
| Reference | WBPaper00036484 | |||
| Transgene | WBTransgene00007882 |
