WormBase Tree Display for Expr_pattern: Expr877
expand all nodes | collapse all nodes | view schema
| Expr877 | Expression_of | Gene | WBGene00001208 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00001208 | ||
| Expression_data | Life_stage | WBls:0000002 | |
| Anatomy_term (15) | |||
| GO_term | GO:0005634 | ||
| Subcellular_localization | nuclei. | ||
| Type | Reporter_gene | ||
| Pattern | GFP was detected in various nuclei starting from before gastrulation through adulthood. Newly hatched larvae expressed GFP in nuclei of the hypodermis (hyp3, hyp4, hyp6, and hyp7), intestine, pharyngeal muscle cells, and neurons (in the head and in the ventral, retrovesicular, preanal, and lumbar ganglia). In the second larval stage (L2), more hypodermal nuclei fluoresced in the body and the tail. In adults, GFP fluorescence was much fainter in the hypodermal cells and no longer delectable in some neurons in the head and tail. Other neuronal expression and the intestinal expression remained. GFP::EGL-44 was expressed in two cell types, the FLP and HSN cells, whose cell fate is altered in egl-44 mutants. The fluorescence in the FLP cells was maintained from the L1 larval stage through adulthood. In contrast to the FLP cells, which expressed the fusion postembryonically, the HSN neurons fluoresced only embryonically, at the 1.5-fold stage. Authors confirmed the identity of these embryonic HSN cells by noting the absence of this fluorescence in egl-1(n487) mutants, animals in which the HSN neurons die at this time. GFP::EGL-44 expression was not seen in the touch cells. | ||
| Picture | WBPicture0000008414 | ||
| Remark | A C-terminal GFP fusion (EGL-44::GFP) fluoresced in pharyngeal muscle cells and some intestinal nuclei. Because this reporter did not rescue the Egl phenotype, it may not reflect accurately the egl-44 expression pattern. A free C terminus may be important for function, because an N-terminal protein fusion (GFP::EGL-44) partially rescued the Egl phenotype, and authors believe the pattern of fluorescence is more likely to represent the true expression pattern of the gene. | ||
| Reference | WBPaper00004624 | ||
| Transgene | WBTransgene00030699 |
