WormBase Tree Display for Expr_pattern: Expr8588
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| Expr8588 | Expression_of | Gene | WBGene00006747 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006747 | |||
| Expression_data | Anatomy_term | WBbt:0003675 | Certain | |
| WBbt:0003679 | Certain | |||
| GO_term | GO:0045202 | |||
| Subcellular_localization | Double immunolabeling with various presynaptic markers. Endogenous UNC-7 did not colocalize with active zone proteins UNC-10 and SYD-2. No colocalization were observed between UNC-7 and presynaptic GFP markers, GFP::SYD-2 or SNB-1::GFP, expressed in GABAergic neurons. Instead, many UNC-7 puncta are perisynaptic, closely flanking presynaptic markers. Also, UNC-7 puncta were not directly apposed to postsynaptic UNC-49::GFP puncta but appeared perisynaptic, further suggesting that the many small UNC-7 puncta flank presynaptic regions. | |||
| Type | Antibody | |||
| Pattern | Whole-mount immunofluorescent staining of wild-type animals with an antibody against UNC-7 revealed specific staining in both the nervous system and muscle. Along the processes of dorsal and ventral nerve cords, UNC-7 localizes to many small discrete puncta interspersed by infrequent larger puncta. Antibody staining against UNC-7 in a strain expressing a postsynaptic GABA receptor marker, UNC-49::GFP (oxIs22), further confirmed that UNC-7 puncta is present along nerve processes apposed to muscle. UNC-7 also localizes to discrete puncta outlining the surface of body-wall muscles, mostly at posterior muscles, and along muscle arms that extend perpendicular from toward the nerve cords. | |||
| Picture | WBPicture0000011158 | |||
| Remark | Picture: Figure 4, Figure S2A. | |||
| Reference | WBPaper00033075 | |||
| Antibody_info | WBAntibody00001906 |
