WormBase Tree Display for Expr_pattern: Expr7897
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| Expr7897 | Expression_of | Gene | WBGene00004457 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004457 | |||
| Expression_data | Anatomy_term | WBbt:0005735 | Certain | |
| GO_term | GO:0043005 | |||
| Subcellular_localization | Dynamin is associated with synaptic vesicles and is required for endocytosis. DYN-1::GFP and RPM-1 showed an adjacent, nonoverlapping, distribution. Thus, endogenous RPM-1 is localized in a region that is close to, but not overlapping with, synaptic vesicle release and recycling domains. | |||
| In the nerve ring and nerve cords, endogenous expression of RPM-1 was mostly punctate, with varying degrees of intensity. No signal was detected in the cell bodies. | ||||
| Within the nerve ring and dorsal and ventral nerve cords, anti-RPM-1 signal was found to be close to, but not overlap with, those of anti-RIM or anti-SNT-1. Measurement of the average distance between RPM-1 and RIM puncta in the dorsal cords showed that 75% of RPM-1 puncta were within 250 nm of a RIM puncta (n = 100 RPM-1 puncta). Anti-SNT-1 or anti-RIM staining showed a string of fluorescent puncta spaced evenly along the SAB axons, while anti-RPM-1 staining was seen adjacent to those of anti-RIM or anti-SNT-1. Moreover, anti-RPM-1 signal showed a graded pattern such that higher immunofluorescent intensity was consistently detected at the anterior ends of the SAB axons. | ||||
| Type | Antibody | |||
| Pattern | RPM-1 expression was seen predominantly in the nervous system. The specificity of the RPM-1 antibody was confirmed by staining rpm-1 mutant animals. | |||
| Remark | Picture: Figure 1. | |||
| Reference | WBPaper00031466 | |||
| Antibody_info | WBAntibody00001369 |
