WormBase Tree Display for Expr_pattern: Expr668
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| Expr668 | Expression_of | Gene | WBGene00001174 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00001174 | ||
| Expression_data | Life_stage | WBls:0000024 | |
| WBls:0000003 | |||
| WBls:0000038 | |||
| WBls:0000027 | |||
| WBls:0000035 | |||
| WBls:0000041 | |||
| Anatomy_term (45) | |||
| GO_term | GO:0005634 | ||
| Subcellular_localization | Expressed in the nuclei. | ||
| Type | Antibody | Anti-EGL-5 antibodies were raised in rabbits by injection of a ST:EGL-5 fusion protein isolated from Escherichia coli (HRP, Inc., Denver, PA). For generation of the fusion protein, the coding sequence of a full-length egl-5 cDNA was used. | |
| Reporter_gene | |||
| Pattern | Postembryonic expression is observed in the rectum epithelium. A major site of EGL-5 expression is in the rectal epithelium. At hatching, the rectal expression is in K, F, B, U and Y. In addition expression is seen in (Y differentiates into) PDA motor neuron, (K divides to rise to) part of dorsal rectal epithelium and a cell that becomes DVB motor neuron. In males male-specific neurons show expression. In males Ab staining is observed in B.a and B.p as well as Y.p and Y.p in L1 and early L2. It appears that most/all B, Y, U, F, K descendants express EGL-5. Ventral neuroblast P12, staining is first seen in P12.a and P12.p in 12-h worms. Staining is maintained in P12 descendants in 15-h until adulthood. Both sexes' mechanosensory neurons, expression is seen in PLM neurons throughout larval development. In addition two cells express EGL-5, one in anterior region of each lumbar ganglion, likely to be PVC interneurons. Both sexes' muscles cells, expression is detected in 4-6 left/right pairs of posterior body-wall muscle cells in L1 larvae at earliest examined time 10-12 h. At L2 staining is detected in 12 left/right pairs of nuclei. Staining is strongest in the most posterior nuclei and tapers of towards the anterior. Staining in posterior body wall muscle cells remains throughout larval development and into adulthood in both sexes. In L3 males, sex-specific muscle lineages and sex-specific muscles stain strongly. These muscles include the diagonal muscles, muscles of spicule, gubernaculum and other sex muscles. Staining in these muscles persist until adulthood. HSN neurons, expression from L1 onwards through to adulthood. Male gonad, first detected in the male gonad in late L1 in a group of 6 cells at the anterior end. It appears that expression is clustered in a region that consists of both somatic cells and germ cells. Later at the beginning of the late mitotic period, staining nuclei lose their clustered arrangement. By 34 h, staining is seen in several dividing cells that form the primordium of the seminal vesicles as well as in two large nuclei in the valve region. In the nuclei of diving cells staining surrounds a condensed chromatin. This pattern persists until the end of the late mitotic period (35-37 h posthatching) when staining is also detected in sperm cells. No staining was observed in cells of the vas deferens. Lateral hypodermis, expression is seen in male seam from mid-L2. Staining first appears in V6.ppp at 20-22 h postembryonic development. Staining persists in V6.pppa and V6.pppp but at a lower level. Intensity of staining increases in R5 and R6 and to lesser degree in R4. Identification of staining cells in ray sublineages was not possible due to intense fluorescence of B-lineage cells lying in the same region. However it was possible to observe expression of a reporter gene in R4, R5, and R6 and also in cells of the R5 and R6 sublineages. | ||
| Remark | This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). | ||
| Reference | WBPaper00003426 | ||
| Transgene | WBTransgene00029690 | ||
| Antibody_info | WBAntibody00000216 |
