WormBase Tree Display for Expr_pattern: Expr603
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| Expr603 | Expression_of | Gene | WBGene00000445 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000445 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| WBls:0000015 | ||||
| WBls:0000021 | ||||
| Anatomy_term | WBbt:0003681 | |||
| WBbt:0003721 | Certain | |||
| WBbt:0003737 | Certain | |||
| WBbt:0003739 | Certain | |||
| WBbt:0003740 | Certain | |||
| WBbt:0003744 | Certain | |||
| Type | Antibody | Rabbit polyclonal antibodies raised separately against GST::CEH-22 and poly-his::CEH-22 affinity purified from E.coli. | ||
| Pattern | Antibody staining limited to nuclei within pharynx and is detected from beginning of morphogenesis onwards. CEH-22 first detected approx. 330 minutes after fertilization (the lima bean stage) in 11-14 pharyngeal muscle nuclei (probably pharyngeal muscle). At 1.5-fold stage, 14-23 pharyngeal nuclei stain. Cells identified as m3, m4, m5 and m7. In embryos that have completed elongation (pretzel stage), CEH-22 positive nuclei are identified as m3, m4, m5 and m7. Also detected in 6 other pharyngeal nuclei, which are believed to be m1 muscles. After hatching, staining persists in m1, m3, m4, m5 and m7 but absent in m6 and m2. | |||
| Picture | WBPicture0000007904 | |||
| WBPicture0000007905 | ||||
| Remark | Identical staining pattern observed with both antisera (ST::CEH-22 and poly-his::CEH-22 ). Reporter_gene X-gal staining identical to anti-CEH-22 antibodies (Construct contains ~4 kb of ceh-22' -flanking DNA fused to lacZ within the ceh-22 5'-UTR.) | |||
| This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). | ||||
| see Expr748 for western analysis data. | ||||
| Reference | WBPaper00002011 | |||
| Antibody_info | WBAntibody00000082 | |||
| WBAntibody00000083 |
