WormBase Tree Display for Expr_pattern: Expr541
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| Expr541 | Expression_of | Gene | WBGene00001609 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00001609 | ||
| Expression_data | Life_stage | WBls:0000007 | |
| WBls:0000008 | |||
| WBls:0000005 | |||
| WBls:0000010 | |||
| Anatomy_term (31) | |||
| GO_term | GO:0005737 | ||
| GO:0016020 | |||
| Subcellular_localization | GLP-1 was found in the cytoplasm at the 2-cell stage, then in cytoplasm and membranes at 4-cell and 8-cell stages. Cytoplasmic GLP-1 fades after the 8-cell stage, and disappears by the 28-cell stage. Membrane-associated GLP-1 is faint by the 28-cell stage. The glp-1 mRNA was distributed uniformly through the 8-cell stage. Levels of glp-1 mRNA decline after the 8-cell stage and largely disappear by the 28-cell stage, though signal consistently persisted later in posterior parts of embryos. mRNA reappears after 100-cell stage, paralleling immunostaining results. | ||
| Type | Antibody | anti-GLP-1 rat polyclonal antibodies | |
| In_situ | antisense probe to glp-1 | ||
| Pattern | Faint expression in AB at 2-cell stage, becoming stronger in AB descendants after 4-cell stage. Signal weakens, between the 8- and 28-cell stages. GLP-1 not detected again until after the 100-cell stage in unidentified cells. | ||
| Picture | WBPicture0000011555 | ||
| Remark | Subcellular localization: GLP-1 was found in the cytoplasm at the 2-cell stage, then in cytoplasm and membranes at 4-cell and 8-cell stages. Cytoplasmic GLP-1 fades after the 8-cell stage, and disappears by the 28-cell stage. Membrane-associated GLP-1 is faint by the 28-cell stage. The glp-1 mRNA was distributed uniformly through the 8-cell stage. Levels of glp-1 mRNA decline after the 8-cell stage and largely disappear by the 28-cell stage, though signal consistently persisted later in posterior parts of embryos. mRNA reappears after 100-cell stage, paralleling immunostaining results. | ||
| early embryo(author) = blastula + gastrulating embryo(curator). | |||
| Experiment | Strain | WBStrain00000001 | |
| Reference | WBPaper00001941 | ||
| Antibody_info | WBAntibody00000090 | ||
| WBAntibody00001538 | |||
| WBAntibody00001539 |
