WormBase Tree Display for Expr_pattern: Expr509
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| Expr509 | Expression_of | Gene | WBGene00004013 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004013 | ||
| Expression_data | Life_stage | WBls:0000038 | |
| WBls:0000035 | |||
| WBls:0000018 | |||
| WBls:0000019 | |||
| WBls:0000020 | |||
| WBls:0000017 | |||
| WBls:0000014 | |||
| WBls:0000021 | |||
| Anatomy_term (12) | |||
| Type | In_situ | Digoxigenin-labeled DNA probes | |
| Reporter_gene | |||
| Pattern | In the gut at the 8E cell stage; E descendants continue to show expression through late embryo. Pharyngeal cells derived from both AB and MS lineages show staining starting at comma stage. Intestinal and pharyngeal staining decline after hatching. The developing somatic gonad shows expression at L3 and L4. In comma to 1.5-fold stage embryos, hybridization was also seen in the tail region. | ||
| Remark | For in situ hybridization, the signal from anti-sense probes was abolished by ribonuclease treatment of embryos. cDNA clone used as template for unidirectional PCR began at position 14 and ended at 2033 to remove the poly-A tract. | ||
| For reporter gene, integrant gave same results, confirming that mosaicism. was not responsible for pattern. Reporter gene results gave resolution of cells. Transgenic Marker: rol-6(su1006). | |||
| Reporter gene and in situ hybridization gave similar results, but lacZ expression was more intense in gut than pharynx. Hybridization results differed in being more intense in pharynx. | |||
| early embryo(author) = late cleavage stage embryo(curator). | |||
| late embryo(author) = comma + 1.5-fold + 2-fold + 3-fold + fully-elongated embryo(curator). | |||
| Experiment | Strain | WBStrain00000001 | |
| Reference | WBPaper00002565 | ||
| Transgene | WBTransgene00029615 |
