WormBase Tree Display for Expr_pattern: Expr4351
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| Expr4351 | Expression_of | Gene | WBGene00001086 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00001086 | ||
| Expression_data | GO_term | GO:0000228 | |
| Subcellular_localization | DPY-27 antibody staining and X chromosome paint are colocalized. In contrast, DPY-27 antibody staining (green) and the chromosome paint for the middle of chromosome III (red) were not colocalized in embryos. | ||
| Type | Antibody | To visualize the distribution of DPY-27 and a painted chromosome in interphase nuclei, authors performed immunofluorescent staining with DPY-27 antibodies followed by hybridization with the chromosome-painting probe. | |
| Picture | WBPicture0000011565 | ||
| WBPicture0000011566 | |||
| WBPicture0000011567 | |||
| Remark | The distribution of the chromosome paints and DPY-27 antibody can be seen more clearly in the large gut nuclei of the adult hermaphrodite. These nuclei are 32-ploid, yet both the DPY-27 antibody staining and chromosome paints are nonetheless seen in restricted domains. By contrast, in favorable optical sections in which the chromosome paint for chromosome Ill and DPY-27 can both be seen, the two do not colocalize. These results were confirmed in a separate experiment with gut nuclei, in which the nuclear location of DPY-27 was detected by indirect immunofluorescence with fluorescein-labeled antibodies, the X chromosome was detected by hybridization of rhodamine-dUTP-labeled paint, and chromosome Ill was detected by hybridization of a Cy5-dCTP-labeled paint. Comparison of the combined DPY-27 and X chromosome images, the combined DPY-27 and chromosome Ill images, and the combined DPY-27, X chromosome, and chromosome Ill images reveals that the X paint and DPY-27 colocalize but occupy a different domain from that of chromosome Ill. | ||
| Reference | WBPaper00002061 | ||
| Antibody_info | WBAntibody00000970 |
