WormBase Tree Display for Expr_pattern: Expr4241
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| Expr4241 | Expression_of | Gene | WBGene00009574 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00009574 | |||
| Expression_data | Anatomy_term | WBbt:0005735 | Certain | |
| Type | Reporter_gene | |||
| Pattern | GFP and CFP expression patterns observed for F40E10.6 reporter fusions generated by fosmid recombineering were strong and clear, restricted to the nervous system, from the circumpharyngeal nerve ring in the head, down the nerve cord, around the vulva, to the tail ganglia. No expression could be detected in the pharynx. | |||
| Remark | The large amounts of flanking DNA in the recombineered fusions may avoid artifactual expression potentially arising from fusions with incomplete promoters. The more tightly localized distribution of the fluorescent proteins may be because the product of the X-gal staining reaction, used to visualize -galactosidase, spreads from the source. However, the recombineered fusions result in the fluorescent tag being added to the terminus of a virtually intact C.elegans protein, and the fusion protein may then show a subcellular distribution more tightly reflecting the distribution of the endogenous protein. | |||
| Reference | WBPaper00027637 | |||
| Transgene | WBTransgene00029066 |
