WormBase Tree Display for Expr_pattern: Expr3784
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| Expr3784 | Expression_of | Gene | WBGene00012484 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00012484 | ||
| Expression_data | GO_term | GO:0005819 | |
| GO:0005737 | |||
| GO:0005783 | |||
| GO:0043186 | |||
| Subcellular_localization | CAR-1 localized diffusely throughout the cytoplasm, weakly to the mitotic spindle in the GFP::CAR-1 strain and strongly to large and small puncta (mean large = 1.0 +/- 0.3 N5m; mean small = 0.5 +/- 0.1 N5m; N = 8 embryos and 40 puncta of each category). Only a few small CAR-1 containing puncta were seen in the one cell embryo and the interphase 2-cell embryo. However, there was a dramatic increase in the number of small puncta during and after the division of the anterior (AB) cell, whereas the diffuse cytoplasmic signal decreased with subsequent cell divisions. The population of these smaller puncta did not noticeably change in a cyclical manner, suggesting that they are not under cell cycle regulation. In addition to the strongly labeled puncta, labeled CAR-1 could be found in the spindle region during mitosis in live C. elegans embryos. The intensity of this signal increased as mitosis progressed and, as the chromosomes separated, the signal extended along the elongating spindle to include the pericentriolar region, resembling the organization of the ER. | ||
| Type | Antibody | ||
| Reporter_gene | |||
| Picture | WBPicture0000008778 | ||
| WBPicture0000008779 | |||
| Reference | WBPaper00026933 | ||
| Transgene | WBTransgene00028873 | ||
| Antibody_info | WBAntibody00000955 |
