WormBase Tree Display for Expr_pattern: Expr3695
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| Expr3695 | Expression_of | Gene | WBGene00021095 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00021095 | ||
| Expression_data | Life_stage | WBls:0000024 | |
| WBls:0000003 | |||
| WBls:0000038 | |||
| WBls:0000027 | |||
| WBls:0000035 | |||
| Anatomy_term (12) | |||
| Type | Reporter_gene | ||
| Pattern | Fluorescence was observed in epithelial cells that synthesize cuticle. Expressed in the hypodermis, including the major body syncytium, hyp7, and hypodermal cells in the head and tail. A pulse of fluorescence was observed in the hypodermis prior to molting. Fluorescence from mlt-8p::gfp-pest was first detected approximately 3 h before the L1/L2 molt, or 13 h after hatchlings synchronized by starvation were fed and incubated at 25 centigrades. The intensity of fluorescence increased until lethargus and then decreased rapidly, such that GFP was barely detectable just 2 h after molting. When monitoring individual transgenic larvae over the course of development, fluorescence from mlt-8p::gfp-pest was observed from 65 +/- 2% to 90 +/- 2% of the duration of each larval stage. The mlt-8 reporter was expressed, in larvae, in a single posterior neuron that remains to be identified. Expression of the gfp fusion genes was never detected in the hypodermis of gravid adults that no longer molt. | ||
| Picture | WBPicture0000007250 | ||
| WBPicture0000007251 | |||
| Reference | WBPaper00026763 | ||
| Transgene | WBTransgene00028823 |
